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To execute this, 200 L of the examined samples, previously diluted with PBS at a 1:5 ratio (v/v), were combined with standard solutions ranging from 0 to 100 mol/L alongside 200 L of blank PBS solution

Moreover, mutations in the Zip7 gene have been linked to a novel autosomal recessive disease characterized by agammaglobulinemia and early-onset infections, highlighting the crucial role of Zip7 in B cell development and immune function, as confirmed through CRISPR-Cas9 mutagenesis in mice [44]